Protein and peptide microarrays

Our technologies improve the conservation of arrayed proteins and provide a high sensitivity to detect molecular interactions.

Examples of our protein and peptide microarray applications:

  • identification of protein interaction partners of bacterial or human origins
  • analysis of regulatory regions of bacterial DNA
  • evaluation of the immune response (AIDS)
  • characterization of allergens in cow’s milk
  • epitope mapping of viral proteins
  • studying of skin proteins

Contact us for your projects

Representative references:
Dupuy L., Gauthier C., Durand G., Musnier A., Heitzler D., Herledan A., Sakanyan V., Crépieux P, Reiter E. A highly sensitive near-infrared fluorescent detection method to analyse signalling pathways by reverse-phase protein array. Proteomics 2009, 9, 5446-5454.

Gaudin J-C., Rabesona H., Choiset Y., Yeretssian G., Chobert J-M., Sakanyan V., Drouet M., Haertlé T. Assessment of the IgE-mediated immune response to milk-specific proteins in allergic patients using microarrays. Clin. Exp. Allergy 2008, 38, 686-693. PDF

Sakanyan  V. High-throughput and multiplexed protein array technology: protein-DNA and protein-protein interactions. J. Chromatogr. B Analyt Technol Biomed Life Sci. 2005, 815, 77-95.

Arnaud M.-C., Gazarian T., Palacios Rodriguez Y., Gazarian K., Sakanyan V. Array assessement of phage displayed peptide mimics of Human Immunodeficiency Virus type 1 gp41 immunodominant epitope: binding to antibodies of infected individuals. Proteomics 2004, 4, 1959-1964.

Snapyan M., Lecocq M., Guével L., Arnaud M.-C., Ghochikyan A., Sakanyan V. Dissecting DNA-protein and protein-protein interactions involved in bacterial transcriptional regulation by a sensitive protein array method combining a near-infrared fluorescence detection. Proteomics 2003, 3, 647-657.

Ghochikyan A., Karaivanova I., Lecocq M., Vusio P., Arnaud M.-C., Snapyan M., Weigel P., Guevel L., Buckle M., Sakanyan V. Arginine operator binding by heterologous and chimeric ArgR repressors from Escherichia coli and Bacillus stearothermophilus. J. Bacteriol. 2002, 184, 6602-6614. PDF