Antibody microarrays

Antibody microarrays allow multiplex analysis of the abundance of proteins and their post-translational modifications in cells, biopsies and biological fluids.

Examples of our antibody microarray applications:

  • assessment of the abundance of target proteins in human serum (breast cancer)
  • protein profiling in biopsies (liver cancer)
  • protein profiling in bovin meat
  • protein phosphorylation in the cells treated by anticancer agents
  • protein acetylation in mouse hypophysis
  • studying signaling pathways
  • transactivation of receptor tyrosine kinases in cancer cells
  • biomarker identification (breast cancer)
  • development of a diagnostic tool for cancer (project ANR PNANO 2007)

Contact us for your projects

Representative references:

Guedj N., Zhan Q., Perigny M., Rautou P.E., Degos F., Belghiti J., Farges O., Bedossa P., Paradis V. Comparative protein expression profiles of hilar and peripheral hepatic cholangiocarcinomas. J. Hepatol. 2009, 51, 93-101. PDF

Bitoum M., Durieux A.C., Prudhon B., Bevilacqua J.A., Herledan A., Sakanyan V., Urtizberea A., Cartier L., Romero N.B., Guicheney P. Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis. Hum. Mutat. 2009, 30, 1419-1427. PDF Supplementary Material

Sakanyan V., Yeretssian G. Near infrared fluorescence detection of antigen-antibody interactions on microarrays. In: Spectral Techniques in Proteomics. Ed. D. Sem, CRC Press, 2007, p. 185-205.

Yeretssian G., Lecocq M., Lebon G., Hurst H., Sakanyan V. Competition on nitrocellulose-immobilized antibody arrays: from bacterial protein binding assay to protein profiling in breast cancer cells. Mol. Cell. Proteomics 2005, 4, 605-617. PDF