This new high-throughput method has been
designed for straightforward and rapid screening of natural and chemical
compound libraries (1). The method is based on non-covalent
immobilization of compounds that allows analysing different type of molecules
on the same surface in a single binding assay.
No chemical reactions to immobilise molecules.
The support is tolerant to solvents (DMSO, DMF). Screening can be performed
against both catalytic site and protein-protein interaction surface of target
proteins. Several proteins can be simultaneously probed in parallel binding
assays. Different approaches have been successfully tested to distinguish between
binding to a druggable site and to other protein regions (promiscuous
interactions). Low soluble compounds can be screened as well.
Sakanyan V.
Eur. Biopharm. Review November,
74-78 (2008)
